Document Type : Original Article

Authors

1 Research Department of Agronomy and Plant Breeding, Agricultural Research Institute, University of Zabol, Zabol, Iran

2 Department of Horticulture, Faculty of Agriculture, Herat University, Herat, Afghanistan

3 Agricultural Biotechnology Research Institute, University of Zabol, Zabol, Iran

10.22034/jpbb.2021.268638.1000

Abstract

A long time has passed since the first experience of In Vitro propagation of olive, but the presence of very strong end dominance in the neoplasm stage, which cannot be controlled by various cytokinin treatments, has limited the possibility of propagation in vitro. Therefore, the aim of the present study was to evaluate the micro-propagation feasibility inside olive glasses of olive cultivar Kroneiki. For branching and rooting of lateral buds, the experiment was carried out as a factorial experiment based on completely randomized design with three replications.  Branching factors include five types of culture media (MS, MS1/2, MSM, MSM1/2 and OM), two types of hormones (BAP and Zeatin), and three types of hormone concentrations (control, 0.5 and 1 mg/L) and there were three types of time periods. Rooting factors include five types of culture media (MS, MS1/2, MSM, MSM1/2 and OM), two types of hormone combinations (IBA and NAA), four types of hormone concentrations (control, combination of 0.2 with 0.5, combination 0.5 with 1 and a combination of 0.75 with 1.5 mg/L) and three types of time intervals. Analysis of variance was performed by Statistix10 software and the comparison of mean traits was performed using the least significant difference test. The effects of culture medium, different hormones and also different concentrations of hormones used in the period, on the number of green leaves, number of yellow leaves, fresh weight and dry weight of seedlings as well as rooting were examined , which were found different (P <0.01). The highest length of branch (1.8 cm) was obtained in MSM culture medium with BAP hormone in the third week. The highest fresh weight (0.06 g), the highest dry weight (0.003 g) and the highest amount of green leaves (55.39%) were obtained from the treatment of MSM medium with 1 ppm of BAP hormone. The highest rooting rate (95.17%) was obtained from the treatment of MS1/2 medium with 1.5 ppm of BAP hormone and 0.75 ppm of NAA hormone in the third week. The most effective culture medium and hormone on branching was MSM culture medium with 1 ppm BAP hormone and on rooting, MS1 culture medium with 1.5 ppm BAP hormone and 0.75 ppm NAA hormone.

Graphical Abstract

Effects of culture medium and plant hormones in organogenesis in olive (CV. Kroneiki)

Keywords

Main Subjects

1. peyvandi m, farahzadi h n, arbabiyan s, hoseynimazinani m. (2010). Effects of medium on somatic embryogenesis of Olea europea L. (cv. Kroneiki). Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 18(1): 93-101.
2. Ghane Golmohamadi F, Hosseini R, Morad Nezhad M. (2019). Investigating the effect of simultaneous application of red LED and sodium dikegulac on shoot regeneration and longitudinal growth of olive (Olea europaea L.) cv. Zard explants. Iranian Journal of Plant Biology, 11(1): 81-96. https://doi.org/10.22108/ijpb.2019.110506.1091
3. Rugini E, Tarini P.  (1986). Somatic embryogenesis in olive tree (Olea europaea L.). Paper presented at the Arbres Fruitiers et Biotechnologies. Paris (France). 14-15 Oct 1986.
4. Jain S M, Ochatt S J. (2010). Protocols for in vitro propagation of ornamental plants (Vol. 589): Springer. https://doi.org/10.1007/978-1-60327-114-1
5. Jokari S, Hedayat M. (2017). Effect of growth regulators on proliferation ber four (Ziziphus spp.) In vitro culture. Journal of Plant Research (Iranian Journal Of Biology), 30(3): 531-540.
6. George E F, Hall M A, De Klerk G-J. (2008). Plant growth regulators II: cytokinins, their analogues and antagonists Plant propagation by tissue culture (pp. 205-226): Springer. https://doi.org/10.1007/978-1-4020-5005-3_6
7. Lone S M, Hussain K, Malik A, Magray M, Hussain S M, Rashid M, Farwah S. (2020). Plant Propagation through Tissue Culture–A Biotechnological Intervention. Int. J. Curr. Microbiol. App. Sci, 9(7): 2176-2190. https://doi.org/10.20546/ijcmas.2020.907.254
8. Kaviani B, Hesar A A, Kharabian-Masouleh A. (2011). In vitro propagation of Matthiola incana (Brassicaceae)-an ornamental plant. Plant Omics J, 4(7): 435-440.
9. Murashige T, Skoog F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum, 15(3): 473-497.
10. Leva A. (2011). Innovative protocol for “ex vitro rooting” on olive micropropagation. Central European Journal of Biology, 6(3): 352-358. https://doi.org/10.2478/s11535-011-0010-3
11. Mencuccini M, Rugini E. (1993). In vitro shoot regeneration from olive cultivar tissues. Plant cell, tissue and organ culture, 32(3): 283-288. https://doi.org/10.1007/BF00042290
12. Ahmed E E, Bisztray G, Velich I. (2002). Plant regeneration from seedling explants of common bean (Phaseolus vulgaris L.). Acta Biologica Szegediensis, 46(3-4): 27-28.
13. Karami M, Bagherieh-Najjar M B, Aghdasi M. (2013). Optimization of conditions suitable for bean (Phaseolus vulgaris L.) regeneration. Journal of Plant Biology, 5(15): 1-14.
14. Reis E, Batista M T, Canhoto J M. (2008). Effect and analysis of phenolic compounds during somatic embryogenesis induction in Feijoa sellowiana Berg. Protoplasma, 232(3-4): 193-202. 10.1007/s00709-008-0290-2
15. Sobhanizadeh A, Solouki M, Fazeli Nasab B. (2016). Kinetin impact on the growth rate of Black Cumin under salt stress. Secound International Conference on Agriculture, Natural Resources, Environment and medicinal plants, Iran.
16. Shahadati-Moghadam Z. (2001). Plant growth regulators. master's seminar, the University of Mazandaran.  
17. Fazeli-Nasab B, Masour O, Mehdi A. (2012). Estimate of callus induction and volume immature and mature embryo culture and respons to in-vitro salt resistance in presence of NaCL and ABA in salt tolerant wheat cultivars. Int. Agric. Crop Sci, 4(1): 8-16.
18. Elaleem K G A, Modawi R S, Khalafalla M M. (2009). Effect of plant growth regulators on callus induction and plant regeneration in tuber segment culture of potato (Solanum tuberosum L.) cultivar Diamant. African journal of biotechnology, 8(11).
19. JayaSree T, Pavan U, Ramesh M, Rao A, Reddy K J M, Sadanandam A. (2001). Somatic embryogenesis from leaf cultures of potato. Plant cell, tissue and organ culture, 64(1): 13-17.
20. Ahmad N, Fazal H, Zamir R, Khalil S A, Abbasi B H. (2011). Callogenesis and shoot organogenesis from flowers of Stevia rebaudiana (Bert.). Sugar tech, 13(2): 174-177.
21. Hohtola A. (1988). Seasonal changes in explant viability and contamination of tissue cultures from mature Scots pine. Plant cell, tissue and organ culture, 15(3): 211-222. doi: 10.1007/BF00033645
22. Taha H, El-Bahr M, Seif-El-Nasr M. (2009). In vitro studies on Egyptian Catharanthus roseus (L.) G. Don. IV: manipulation of some amino acids as precursors for enhanced of indole alkaloids production in suspension cultures. Australian Journal of Basic and Applied Sciences, 3(4): 3137-3144.
23. Hussain Z, Khan M H, Bano R, Rashid H, Chaudhry Z. (2010). Protocol optimization for efficient callus induction and regeneration in three Pakistani rice cultivars. Pak. J. Bot, 42(2): 879-887.
24. Piri K h, Nazarian F. (2001). Plant Tissue Culture. Abu Ali Sina University Press.: 352 Pages.
25. Saravanan S, Nadarajan N. (2005). Effect of Media Supplements on in vitro response of Sesame (Sesamum indicum L.) Genotypes. Res J Agric Biol Sci, 1: 98-100.
26. Baskaran P, Jayabalan N. (2006). In vitro mass propagation and diverse callus orientation on Sesamum indicum L. an important oil plant. Journal of Agricultural Technology, 2: 259-269.
27. Rodriguez R. (1982). Callus Induction and Root-Formation from Invitro Culture of Walnut Cotyledons. HortScience, 17(2): 195-196.
28. Mehrabi A-A, Fazeli-Nasab B. (2012). In vitro culture of Allium scorodoprasum spp. Rotundum: callus induction, somatic embryogenesis and direct bulblet formation. Intl. J. Agri. Crop Sci, 4(1): 1-7.
29. Fazelienasab B, Omidi M, Amiritokaldani M. (2004). Effects of abscisic acid on callus induction and regeneration of different wheat cultivars to mature embryo culture. News directions for a diverse planet: Proceedings of the 4th International Brisbane, Australia, 26.
30. Sobhanizade A, Solouki M, Fazeli-Nasab B. (2017). Optimization of callus induction and effects of biological and non-biological elicitors on content of phenol/flavonoid compounds in Nigella sativa under in-vitro conditions. Cell and Tissue Journal, 8(2): 165-184.
31. Murashige T. (1980). Plant growth substances in commercial uses of tissue culture Plant Growth Substances 1979 (pp. 426-434): Springer.
32. Ahmadi A, Kavoosi M R, Soltanloo H, Salehi Jozani G, Sattarian A. (2019). Investigating callus induction and regeneration of Zelkova carpinifolia forest species In vitro. Journal of Plant Research (Iranian Journal Of Biology), 32(1): 16-27.
33. Motaghi M, Mokhtari A. (2019). The determination of optimal condition for micro propagation of Cratagus aronia under in vitro culture. Journal of Plant Research (Iranian Journal Of Biology), 32(1): 39-51.
34. Fazelienasab B, Omidi M, Amiritokaldani M. (2004). Effects of abscisic acid on callus induction and regeneration of different wheat cultivars to mature embryo culture.-4th Int. Crop Sci. Congr. Brisbane, Australia, 26.
35. Fazeli-Nasab B, Omidi M, Amiritokaldani M. (2012). Callus induction and plant regeneration of wheat mature embryos under abscisic acid treatment. Int J Agric Crop Sci, 4: 17-23.
36. Gökbunar L. (2007). In vitro micropropagation of hawthorn (Crataegus sp.). University of Kahramanmaras Sutcu Imam Institute of Natural and Applied Sciences, Department of Horticulture (M.sc Thesis), 42.  
37. Singh A, Reddy M P, Chikara J, Singh S. (2010). A simple regeneration protocol from stem explants of Jatropha curcas—a biodiesel plant. Industrial Crops and products, 31(2): 209-213. https://doi.org/10.1016/j.indcrop.2009.10.007
38. Donmez A A. (2004). The genus Crataegus L.(Rosaceae) with special reference to hybridisation and biodiversity in Turkey. Turkish Journal of Botany, 28(1-2): 29-37.
39. Bujarska-Borkowska B. (2002). Breaking of seed dormancy, germination and seedling emergence of the common hawthorn (Crataegus monogyna Jacq.). Dendrobiology, 47(47 Supplement): 61-70.
40. Sharma S, Ramamurthy V. (2000). Micropropagation of 4-year-old elite Eucalyptus tereticornis trees. Plant Cell Reports, 19(5): 511-518. https://doi.org/10.1007/s002990050765
41. Negahdar N. (2019). Micropropagation of Buxus hyrcana Pojark., an ornamental species under danger of extinction. Journal of Plant Research (Iranian Journal Of Biology), 32(2): 332-342.
42. Khawar K, Sarhin E, Sevimay C, Cocu S, Parmaksiz I, Uranbey S, Ipek A, Kaya M, Sancak C, Ozcan S. (2005). Adventitious shoot regeneration and micropropagation of Plantago lanceolata L. Periodicum Biologorum, 107(1): 113-116.
43. Neibaur I, Gallo M, Altpeter F. (2008). The effect of auxin type and cytokinin concentration on callus induction and plant regeneration frequency from immature inflorescence segments of seashore paspalum (Paspalum vaginatum Swartz). In Vitro Cellular & Developmental Biology-Plant, 44(6): 480.
44. Schultz W, Hose S, AbonMandou R A, Czygan F C. (1990). Melissa officinalis L. (Lemon balm), Invitro culture and the production and analysis of volatile compounds. Biotechnology in agriculture and forestry, 24: 242.