Document Type : Original Article

Authors

1 Agricultural Biotechnology Research Institute, University of Zabol, Zabol, Iran

2 Assistant Professor, Department of Biology, Payam Noor University, Ilam, Iran

3 PhD Student, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran

10.22034/jpbb.2021.127686

Abstract

Background: In vitro cultivation method, in addition to being a suitable method for propagating commercial rose cultivars with very high uniformity and in a short time. Today, it is considered as a desirable solution for correction through genetic correctness.
Methods: In order to study the effect of hormone and culture medium on the micropropagation potential of Golmohammadi, an experiment was conducted in 2020 in the tissue culture laboratory of Zabol University Biotechnology Center in a completely randomized design with three replications. In this study, 5 culture media including MS, 2.1MS, VS, 1.2VS and WPM with four hormones benzylaminopurine (BAP), 2-isopentenyl adenine (2iP), naphthalene acetic acid (NAA) and indole butyric acid (IBA) with concentrations of -0, 0.5, 1 and 1.5 mg/l were evaluated.
Results: The results of analysis of variance showed a significant difference between culture media, type and causality of hormones and their interaction for the studied traits. The best response for the studied traits was obtained from VS medium and the weakest response was obtained from WPM medium. The results of interaction showed that the highest values ​​for shoot length, greenness, percentage of yellow leaves, fresh and dry weight of shoots in VS medium were obtained using BAP hormone at a concentration of 1.5 mg / l.
Conclusion: The highest mean rooting percentage, fresh and dry weight of roots were obtained from VS medium using a concentration of 1.5 mg/l IBA.

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