Document Type : Original Article


1 Research Department of Agronomy and Plant Breeding, Agricultural Research Institute, University of Zabol, Zabol, Iran

2 Department of Horticulture, Faculty of Agriculture, Herat University, Herat, Afghanistan

3 Agricultural Biotechnology Research Institute, University of Zabol, Zabol, Iran



Background: A long time has passed since the first experience of In Vitro propagation of olive, but the presence of very strong end dominance in the neoplasm stage, which cannot be controlled by various cytokinin treatments, has limited the possibility of propagation in vitro. Therefore, the aim of the present study was to evaluate the micro-propagation feasibility inside olive glasses of olive cultivar Kroneiki.
Methods: For branching and rooting of lateral buds, the experiment was performed factorially in a completely randomized design with three replications.  Branching factors include five types of culture media (MS, MS1/2, MSM, MSM1/2 and OM), two types of hormones (BAP and Zeatin), and three types of hormone concentrations (control, 0.5 and 1 mg/l) and there were three types of time periods. Rooting factors include five types of culture media (MS, MS1/2, MSM, MSM1/2 and OM), two types of hormone combinations (IBA and NAA), four types of hormone concentrations (control, combination of 0.2 with 0.5, combination 0.5 with 1 and a combination of 0.75 with 1.5 mg/l) and three types of time intervals. Analysis of variance was performed by Statistix10 software and the comparison of mean traits was performed using the least significant difference test.
Results: The effect of culture medium, different hormones and also different concentrations of hormones used in the period, on the number of green leaves, number of yellow leaves, fresh weight and dry weight of seedlings as well as rooting were different (P <0.01). The highest length of branch (1.8 cm) was obtained in MSM culture medium with BAP hormone in the third week. The highest fresh weight (0.06 g), the highest dry weight (0.003 g) and the highest amount of green leaves (55.39%) were obtained from the treatment of MSM medium with 1 ppm of BAP hormone. The highest rooting rate (95.17%) was obtained from the treatment of MS1/2 medium with 1.5 ppm of BAP hormone and 0.75 ppm of NAA hormone in the third week.
Conclusion: The most effective culture medium and hormone on branching were MSM culture medium with 1 ppm BAP hormone and on rooting, MS1 culture medium with 1.5 ppm BAP hormone and 0.75 ppm NAA hormone were obtained, respectively.


Main Subjects

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