Document Type : Original Article

Authors

1 M.Sc. Student of medicinal Plants, graduated from university of Zabol, Zabol, Iran

2 Associate professor, Dep. Of Plant Breeding and Biotechnology, Universe of Zabol, Zabol, Iran

3 Research Dep. Of Agronomy and Plant Breeding, Agricultural Research Institute, university of Zabol, Zabol, Iran

10.22034/jpbb.2021.122161

Abstract

Background: The genetic diversity study of mangoes for breeding programs and conservation of genetic resources is vital and knowledge of genetic diversity in Mango genotypes is important for selecting parents in breeding programs. Therefore, genetic variation of 30 genotypes of Iranian mango plants were evaluated using 15 IRAP markers.
Methods: The plant genotypes were obtained from Jiroft region. DNA extraction was performed by the CTAB method and primers were designed using the primers3 database. The data were analyzed using the GenAlex and Popgene softwares.
Results: The number of bands ranged from 3 (Ltr3 and Ltr1 & Ltr15) to 12 (Ltr1 & Ltr2) for each marker. The highest (4.76) and lowest (0.18) marker indexes were found for the markers Ltr2, and Ltr15, respectively. The marker indexes average was 1.91. The highest number of effective alleles (1.79), neon variation index (0.43) and Shannon variety (0.63) were belonging to the primer of Ltr3. The least heterozygosity(12.2), polymorphic location(36.36), Shannon variety index (17.1) and effective alleles number (18.1) were belonging to the mangoes collected from Rodan. The highest Heterozygosity(28.8), multivariate percentage(90.91), Shannon diversity index (41.4) and effective alleles (43.1) were belonging to the mangoes collected from Manojan region. The molecular variance analysis showed that the genetic diversity between populations was 90 percent. The highest genetic distance was found between mango and coconut genotypes. The highest intra-population variation was found in among all genotypes of Jiroft area.
Conclusion: it can be concluded that for any kind of crossing and modification of the medicinal plant mango, it is better to use the genotypes of Jiroft region, especially almutri as one of Paternal, maternal or genetic donors.

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